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(6.10)

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range, which is relatively slow, but logic is massively parallel, and therefore the end result is actually more ef cient than a modern dissipative machine. Once started, the logic requires practically no power, a feature suggested by the neural models in this book, and hence this novel design may rightly be claimed to be an adiabatic computer. An adiabatic computer is one whose heat dissipation approaches zero while executing programs, permitting a package smaller than would otherwise be possible. It is hoped that this book will inspire applications, not just in computer design, but in related elds such as medicine, logic devices, robotics, arti cial intelligence, neuroscience, and last but not least, education. Modeling is important to any science, but it is especially important to neuroscience, because these areas have a vast and exponentially growing quantity of data, the taking of which has immeasurably outpaced the development of models to explain and digest this explosion of information. Without adequate models, scienti c progress would be dif cult to impossible.

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ity for self-association Thus, despite the continued search for the hole de nition in terms of a de ned architecture, the possibility of oligomers of varying size and shape displaying full functionality will remain 53 Pinholins: A Simple Membrane Depolarization Is Enough Although holins function to produce a membrane lesion to allow the passage of a medium-sized globular protein, in some cases the holins function simply to activate the endolysin (Park et al 2007) Such activation consists in the release from the cytoplasmic membrane into the periplasm of the endolysin molecule which is synthesized as translocated but in a membrane-tethered inactive form (Xu et al 2004, 2005; Park et al 2007) These holins are thus designated as pinholins for differentiating purposes and the corresponding endolysins referred to as signal-anchor release (SAR) endolysins.

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At physiological levels of expression, the pinholins as S21 are lethal and can mediate host lysis when co-expressed with cognate or noncognate SAR endolysins but not with cytoplasmic endolysins (Park et al 2007) The lysis capacity limit has been shown to be related to the restriction of the membrane permeation process; pinholins depolarize membranes, making them permeable to small ions but not to macromolecules (Park et al 2007) 54 Holins in Action: Sculpturing the Membrane Lesion The recent structural characterization of the S holin chain in detergents has provided a solid argument for building models focused on the very high tendency for the formation of large two-dimensional aggregates in the membrane as the basis for the massive membrane damage Together with this main property, the experimental evidence supporting the occurrence of two types of aggregates differing in size and membrane effects has provided more detail to the models.

ware engineering practices that best support efficient software development manufacturing . In the business component approach, we adopt the classification and approach to best practices defined by the Capability Maturity Model (CMM) of the Software Engineering Institute (SEI), t with its five maturity levels of the CMM, as illustrated in Figure 13 .3 . Component development generally requires at least that an organization is at level 2, and preferably at or approaching level 3 . Adoption of the business component approach as illustrated in this

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